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KMID : 1134819970260061246
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Journal of the Korean Society of Food Science and Nutrition
1997 Volume.26 No. 6 p.1246 ~ p.1251
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Effect of Sexual Pheromone on Phosphorylation of Membrane Protein in Heterobasidiomycetous Yeast Rhodosporidium toruloides
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Jeong Yong-Kee
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Abstract
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When the membrane protein fraction of mating type a cells of heterobasidiomycetous yeast R. toruloides was phosphorylated in vitro, two phosphorylated proteins of 72Kd and 57Kd were detected on SDS-polyacryamide gel. The phosphorylation reaction was inhibited by rhodotorucine A(Rh. A) which is a sexual pheromone secreted by mating type A cells. The inhibition of phosphorylation by Rh.A was dependent on Ca^(2+), and independent on Mg^(2+) or calmodulin. When adding trigger peptidase (TPase) inhibitor, antipain, no inhibition of phosphorylation was observed. Also, by adding the trypsin-digested product of Rh. A, the phosphorylation was inhibited as the action of Rh. A. From these results, it is expected that the inhibition of membrane protein phosphorylation should be caused by the digested product of Rh. A with TPase.
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KEYWORD
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phosphorylation, membrane protein, rhodotorucine A, heterobasidiomycetous yeast, Rhodosporidium toruloides
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